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A PCR test for detecting Escherichia coli O157:H7 based on the identification of the small inserted locus (SIL O157 )
Author(s) -
Perelle S.,
Fach P.,
Dilasser F.,
Grout J.
Publication year - 2002
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2002.01684.x
Subject(s) - biology , escherichia coli , locus (genetics) , serotype , polymerase chain reaction , microbiology and biotechnology , dna , pathogenicity island , genetics , typing , dna profiling , gene
Aims: This paper provides identification of a DNA sequence derived from Shiga toxin‐producing Escherichia coli (STEC) O157:H7 and information on its utilization for detecting STEC O157 by PCR. Methods and Results: Random Amplified Polymorphic DNA and DNA library were used to identify in STEC O157:H7 (strain EDL 933) a 2634‐bp S mall I nserted L ocus, designated SIL O157 . Analysis of 211 bacterial strains showed that the PCR assays amplifying the SIL O157 region could be used to detect STEC O157 with a good specificity. Conclusions: Characterization of a novel locus in STEC O157 is attractive since the serotype O157:H7 of STEC is still by far the most important serotype associated with more serious diseases. This island encodes putative proteins and especially one that is predicted to be an outer membrane protein designated IHP1. Significance and Impact of the Study: Further investigations could now be developed to appreciate the role of the SIL O157 in pathogenicity.