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Production of a monoclonal antibody against Aeromonas hydrophila and its application to bacterial identification
Author(s) -
Delamare A.P.L.,
Echeverrigaray S.,
Duarte K.R.,
Gomes L.H.,
Costa S.O.P.
Publication year - 2002
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2002.01603.x
Subject(s) - aeromonas hydrophila , monoclonal antibody , biology , microbiology and biotechnology , antibody , vibrionaceae , aeromonas , bacteria , virology , immunology , genetics
Aims: to develop a monoclonal antibody (MAb) for the rapid detection of Aeromonas hydrophila in human faeces. Methods and Results: A monoclonal antibody with strong specificity against Aer. hydrophila was obtained by the fusion of myeloma cells and splenocytes of a mouse immunized with vegetative cells of Aer. hydrophila ATCC 7966, followed by a two‐step selection against other species of the genera. ELISA analyses revealed that MAb 5F3 strongly reacts with all the Aer. hydrophila strains evaluated, showing a just basal reactivity against other species of the genera, especially Aer. sobria and Aer. caviae . Conclusions: MAb 5F3 was characterized as an IgG that recognized a polypeptide of approximately 110 kDa. Significance and Impact of the Study: This MAb could be used to detect Aer. hydrophila in human stool samples.