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Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences
Author(s) -
Pavlova S.I.,
Kilic A.O.,
Kilic S.S.,
So J.S.,
NaderMacias M.E.,
Simoes J.A.,
Tao L.
Publication year - 2002
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2002.01547.x
Subject(s) - biology , phylotype , microbiology and biotechnology , lactobacillus crispatus , lactobacillus , 16s ribosomal rna , probiotic , lactobacillus gasseri , phylogenetic tree , lactobacillus paracasei , genbank , lactobacillus rhamnosus , lactobacillus fermentum , ribosomal rna , bacteria , genetics , gene , lactic acid , lactobacillus plantarum
Aims: Lactobacilli are widely distributed in food and the environment, and some colonize the human body as commensal bacteria. The aim of this study was to determine the species of lactobacilli that colonize the vagina and compare them with those found in food and the environment. Methods and Results: Thirty‐five Lactobacillus strains from women from seven countries were isolated, and sequences from 16S rRNA genes were determined and compared with existing data in GenBank. A phylogenetic tree was achieved using the Neighbour‐Joining method based on the analysis of 1465 nucleotides. The results showed that most vaginal isolates were L. crispatus , L. jensenii and L. gasseri . Some were L. vaginalis , L. fermentum , L. mucosae , L. paracasei and L. rhamnosus . Two isolates from a native American woman displayed distinct branches, indicating novel phylotypes. Few vaginal isolates matched food or environmental Lactobacillu s species. Conclusions: Most women worldwide were colonized by three common Lactobacillus species: L. crispatus , L. jensenii and L. gasseri . Significance and Impact of Study: Knowledge of vaginal Lactobacillus species richness and distribution in women worldwide may lead to the design of better probiotic products as bacterial replacement therapy.