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Persistence of Escherichia coli , Salmonella choleraesuis , Aujeszky’s Disease virus and Blue Eye Disease virus in ensilages based on the solid fraction of pig faeces
Author(s) -
MartínezGamba R.,
PradalRoa P.,
Castrejón F.P.,
Herradora M.,
Galván E.,
Mercado C.
Publication year - 2001
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2001.01439.x
Subject(s) - feces , biology , salmonella , virus , inoculation , persistence (discontinuity) , microbiology and biotechnology , escherichia coli , virology , bacteria , immunology , biochemistry , genetics , geotechnical engineering , gene , engineering
Aims: This study was carried out to determine the survival time of Escherichia coli , Salmonella choleraesuis , Aujeszky’s Disease virus and Blue Eye Disease virus in ensilages based on the solid fraction of pig faeces. Methods and Results: The four micro‐organisms were inoculated into microsilos based on the solid fraction of pig faeces, sorghum and molasses. They were left for 0, 7, 14, 28 and 56 days, after which the state of each microsilo was evaluated, and isolation of the inoculated agents was attempted. The four inoculated agents were isolated only on day 0 of ensilage. The viral agents were identified through the cytopathic effect and fluorescence. Conclusions, Significance and Impact of the Study: It is concluded that ensilages based on the solid fraction of pig faeces appear to reduce the risk of the transmission of the agents inoculated in this study and help to reduce the environmental impact by using the solid in animal feed.