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Partial characterization of a bacteriocin produced by Lactobacillus delbrueckii subsp. lactis UO004, an intestinal isolate with probiotic potential
Author(s) -
Boris S.,
JiménezDíaz R.,
Caso J.L.,
Barbés C.
Publication year - 2001
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2001.01403.x
Subject(s) - bacteriocin , probiotic , lactobacillus , lactobacillaceae , microbiology and biotechnology , biology , bacteria , lactobacillus delbrueckii subsp. bulgaricus , lactic acid , fermentation , molecular mass , antimicrobial , chemistry , biochemistry , enzyme , genetics
Aims: The partial characterization of a bacteriocin produced by a human Lactobacillus delbrueckii isolate with probiotic potential. Methods and Results: A bacterocin, UO004, was partially purified by cation exchange followed by a hydrophobic interaction column, biochemically characterized and the N‐terminal region sequenced. Bacteriocin UO004 was found to be a hydrophobic, heat‐stable polypeptide with an apparent molecular mass of 6 kDa. It was also stable and active over a wide pH range. Conclusions: The active compound was proteinaceous, heat‐stable, and had a bactericidal (and bacteriolytic) mode of action on a limited number of micro‐organisms. Such a narrow spectrum of activity is typical for bacteriocins produced by intestinal Lactobacillus . Significance and Impact of the Study: Bacteriocin UO004 from a probiotic strain is a new compound that does not share any homology with any other known lactic acid bacteria bacteriocin. Furthermore, Lact. delbrueckii is regarded as a suitable starter for the production of fermented milks.