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The effects of surface roughness and type of denture acrylic on biofilm formation by Streptococcus oralis in a constant depth film fermentor
Author(s) -
Morgan T.D.,
Wilson M.
Publication year - 2001
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2001.01338.x
Subject(s) - streptococcus oralis , biofilm , surface roughness , streptococcus mutans , dentistry , microbiology and biotechnology , medicine , library science , materials science , composite material , biology , computer science , bacteria , genetics
Aims: To investigate the effects of surface roughness and type of denture acrylic on the early development of a Streptococcus oralis biofilm in a constant depth film fermentor (CDFF). Methods and Results: Streptococcus oralis was incubated with acrylic of known surface roughness in the CDFF. Adherent Strep. oralis were enumerated by viable counting. Cold‐cure acrylic was rougher ( P < 0·01) than heat‐cure acrylic after polishing with abrasive paper of any given grit‐grade. Heat‐cure acrylic was colonized by fewer ( P < 0·001) bacteria than cold‐cure acrylic at any given surface roughness. The number of bacteria adhering to heat‐cure and cold‐cure acrylic increased linearly with mean surface roughness after 2 h incubation, the increase being greater ( P < 0·001) for the cold‐cure compared with the heat‐cure acrylic. However, after 4 h incubation, surface roughness appeared to have no effect on the number of adherent bacteria. Conclusions: The type of acrylic used, and its roughness, affect the early stages of biofilm formation by Strep. oralis . Significance and Impact of the Study: Choosing an appropriate type of smooth acrylic could lead to reduced biofilm formation in vivo .