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A simple chemiluminescence‐based method for rapid enumeration of Listeria spp. microcolonies
Author(s) -
Vidon D.J.M.,
Donze S.,
Muller C.,
Entzmann A.,
Andre P.
Publication year - 2001
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2001.01332.x
Subject(s) - chemiluminescence , listeria , listeria monocytogenes , luminol , enumeration , calcein , chemistry , luminescence , polyvinylidene fluoride , microscope , microbiology and biotechnology , chromatography , materials science , membrane , biology , bacteria , optoelectronics , optics , biochemistry , physics , mathematics , combinatorics , genetics
D.J.‐M. VIDON, S. DONZE, C. MULLER, A. ENTZMANN AND P. ANDRE. 2001. Aims:Listeria monocytogenes is capable, under certain conditions, of producing chemiluminescence which is amplified by luminol. This property was used to detect and count microcolonies of Listeria spp. in a few hours, without the use of a microscope. Methods and Results: After trapping Listeria cells on polyvinylidene fluoride membranes, a chemiluminescence mixture was sprayed onto the membrane. The chemiluminescent spots emitted were analysed by a charge‐coupled device camera connected to a data‐processing system, which restored the intensity of the signals into three dimensional images. The intensity of the luminescence of microcolonies was improved by addition of cellobiose, and by brief exposure to u.v. light. Conclusions: Microcolonies of Listeria spp. can be imaged and counted by luminol‐enhanced chemiluminescence with a photon‐counting system. Significance and Impact of the Study: This method can be applied to the rapid detection and counting of Listeria spp. in raw milk.