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Exopolysaccharide production by Propionibacterium acidi‐propionici on milk microfiltrate
Author(s) -
Gorret N.,
Maubois J.L.,
Ghoul M.,
Engasser J.M.
Publication year - 2001
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2001.01306.x
Subject(s) - library science , computer science
Aims: The aims of this work were to evaluate growth and exopolysaccharide (EPS) production properties of Propionibacterium acidi‐propionici DSM 4900 on milk permeate. Methods and Results: Anaerobic growth on milk permeate was only possible if supplemented with yeast extract (YE). Fermentation capacities of the strain were significantly improved by further increasing the supplemented YE. At 5 g l –1 YE, consumption of 45 g l –1 lactose to produce 9 g l –1 biomass, 34 g l –1 organic acids and 0·65 g l –1 EPS was observed. From a kinetic point of view, EPS production occurred during the bacteria growth phase. At the excreted polysaccharide level, the medium showed shear‐thinning behaviour with a relatively high apparent viscosity of up to 30 mPa.s (milli.Pascal.second) at a shear rate of 17 s –1 . Conclusions: EPS production by P. acidi‐propionici DSM 4900 on milk permeate showed promising rheological behaviour of the milk‐derived medium obtained, even at a low production level. Significance and Impact of the Study: A kinetic study on EPS production by a food‐grade bacterium that could be used in situ in alimentation was carried out.