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Comparison of aerobic denitrification under high oxygen atmosphere by Thiosphaera pantotropha ATCC 35512 and Pseudomonas stutzeri SU2 newly isolated from the activated sludge of a piggery wastewater treatment system
Author(s) -
Su J.J.,
Liu B.Y.,
Liu C.Y.
Publication year - 2001
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2001.01265.x
Subject(s) - pseudomonas stutzeri , denitrification , activated sludge , aerobic denitrification , nitrate , sequencing batch reactor , microbiology and biotechnology , wastewater , chemistry , environmental chemistry , food science , biology , denitrifying bacteria , environmental engineering , bacteria , nitrogen , environmental science , organic chemistry , genetics
Aims: This study compares the ability of Thiosphaera pantotropha ATCC 35512 and the newly isolated Pseudomonas stutzeri SU2 to perform aerobic denitrification. Methods and Results: Nitrate‐supplemented basal medium in airtight crimp‐sealed serum bottles containing an atmosphere of 92% oxygen was inoculated with Ps. stutzeri SU2 or T. pantotropha and incubated at 30 °C. During the 92‐h incubation period, aerobic denitrification by Ps. stutzeri SU2 (NO 3 – – N removal 99·24%) was more efficient than that by T. pantotropha (NO 3 – – N removal 27·29%). Conclusions: Pseudomonas stutzeri SU2, which was isolated from the activated sludge of a sequencing batch reactor treating piggery wastewater, rapidly reduced the nitrate to nitrogen gas without nitrite accumulation. The nitrate removal rate of SU2 was 0·032 mmol NO 3 – – N g cell −1 h –1 after 44 h incubation. Significance and Impact of the Study: Pseudomonas stutzeri SU2 can be used in a full‐scale sequencing batch system for efficient in situ aerobic nitrate removal from piggery wastewater.