Premium
Enhancement of glucose oxidase production in batch cultivation of recombinant Saccharomyces cerevisiae : optimization of oxygen transfer condition
Author(s) -
Kapat A.,
Jung J.K.,
Park Y.H.
Publication year - 2001
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2001.01233.x
Subject(s) - aeration , chemistry , glucose oxidase , oxygen , bioreactor , mass transfer coefficient , impeller , mass transfer , biochemistry , chromatography , enzyme , organic chemistry , thermodynamics , physics
A. KAPAT, J.‐K. JUNG AND Y.‐H. PARK. 2001 . Aims: To obtain an optimal combination of agitation speed and aeration rate for maximization of specific glucose oxidase (GOD) production in recombinant Saccharomyces cerevisiae , and to establish a correlation between k L a vis‐à‐vis oxygen transfer condition and specific glucose oxidase production. Methods and Results: The oxygen transfer condition was manifested indirectly by manipulating the impeller speed and aeration rate in accordance with a Central Composite Rotatory Design (CCRD). The dissolved oxygen concentration and the volumetric oxygen transfer coefficient ( k L a ) were determined at corresponding combinations of impeller speed and aeration rate. The maximal specific extracellular glucose oxidase production (3·17 U mg –1 dry cell mass) was achieved when the initial dissolved oxygen concentration was 6·83 mg l –1 at the impeller speed of 420 rev min –1 and at the rate of aeration of 0·25 vvm. It was found out that while impeller speed had a direct effect on the production of enzyme, a correlation between k L a and specific GOD production could not be established. Conclusions: At the agitation speed of 420 rev min –1 and at 0·25 vvm aeration rate, the degree of turbulence and the dissolved oxygen concentration were thought to be optimal both for cellular growth and production of enzyme. Significance and Impact of the Study: The combined effect of agitation and aeration on recombinant glucose oxidase production in batch cultivation has not yet been reported in the literature. Therefore, this study gives an insight into the effect of these two important physical parameters on recombinant protein production. It also suggests that since there is no correlation between k L a and specific production of GOD, k L a should not be used as one of the scale‐up parameters.