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A reconsideration of the evidence for Escherichia coli STa (heat stable) enterotoxin‐driven fluid secretion: a new view of STa action and a new paradigm for fluid absorption
Author(s) -
Lucas M.L.
Publication year - 2001
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2001.01225.x
Subject(s) - secretion , in vivo , biophysics , absorption (acoustics) , in vitro , heat stable enterotoxin , chemistry , enterotoxin , escherichia coli , biology , microbiology and biotechnology , biochemistry , materials science , gene , composite material
1. Summary, 7 2. Introduction, 8 3. Existing model of net fluid transport, 8 4. A new model for net fluid absorption, 10 5. Evidence for the new model for net fluid absorption, 12 5.1 Evidence from altering ambient carbon dioxide levels, 13 5.2 The role of carbonic anhydrase in net fluid absorption, 14 5.3 Evidence from perfusion with antibiotics, 14 5.4 Evidence from chronic animal models of diarrhoeal disease and resection studies, 14 5.5 Evidence from oral rehydration solution studies, 15 5.6 Evidence from numerical calculations, 16 5.7 Evidence from gene product deletion studies, 17 6. Lack of evidence for the present model of Sta‐driven intestinal secretion, 17 6.1 Changes in short circuit current in Ussing chamber studies, 17 6.2 Evidence from T 84 cell studies, 17 6.3 Proxy in vivo systems, 18 6.4 Short duration perfused in vivo loops, 18 6.5 Notional bicarbonate secretion in vivo and in vitro , 19 7. Reconciliation of the new model for fluid absorption with STa action, 21 7.1 Experimentally based evidence, 21 7.2 Clinical evidence from enzyme deletion, 22 8. Acknowledgements, 23 9. References, 23