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Evaluation of numerical analyses of RAPD and API 50 CH patterns to differentiate Lactobacillus plantarum , Lact. fermentum , Lact. rhamnosus , Lact. sake , Lact. parabuchneri , Lact. gallinarum , Lact. casei , Weissella minor and related taxa isolated from kocho and tef
Author(s) -
Nigatu A.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01202.x
Subject(s) - lactobacillus fermentum , biology , food science , lactic acid , lactobacillaceae , rapd , lactobacillus , microbiology and biotechnology , fermentation , bacteria , lactobacillus plantarum , genetics , medicine , population , environmental health , genetic diversity
Aim: The study was carried out to assess the agreement of API 50 CH fermentation data of food lactobacilli with their RAPD profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. Methods and Results: API 50 CH, RAPD and DNA:DNA reassociation data for 42 lactobacilli from tef and kocho were compared with 30 type strains. Discrepancies were observed between the three methods in assigning strains of Lactobacillus plantarum , Lact. fermentum , Weissella minor and Lact. gallinarum , and Lact. fermentum , Lact. amylophilus, Lact. casei subsp. pseudoplantarum and Lact. rhamnosus. DNA reassociation data agreed well with RAPD results. Conclusions: API 50 CH profiles should be complemented with molecular genetic results for effective identification in Lactobacillus . Significance and Impact of the Study: The study suggested less dependability of metabolic data alone as an identification tool.