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An indirect immunofluorescent antibody technique for detection and enumeration of Vibrio vulnificus serovar E (biotype 2): delevopment and applications
Author(s) -
MarcoNoales E.,
Biosca E.G.,
Milán M.,
Amaro C.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01156.x
Subject(s) - vibrio vulnificus , serotype , enumeration , microbiology and biotechnology , biology , bacteria , vibrio , vibrionaceae , antibody , vibrio infections , specific antibody , immunology , genetics , mathematics , combinatorics
The applications of an indirect fluorescent antibody technique (IFAT), developed to detect and enumerate the pathogenic bacterium Vibrio vulnificus serovar E from water and clinical samples, are described. This technique proved accurate for detecting V. vulnificus , even under starvation conditions and in the non‐culturable state, and could differentiate this species from other bacteria which share the same habitats. The IFAT was succesfully used to diagnose vibriosis from naturally‐ and artificially‐infected eels. The overall data suggest that applying this technique properly in environmental and epidemiological/epizootiological studies could significantly increase our knowledge of this bacterium.