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Direct microscopy of Bacillus endospore germination in soil microcosms
Author(s) -
Thrane C.,
Olsson S.,
Wolstrup J.,
Sørensen J.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01155.x
Subject(s) - endospore , paenibacillus polymyxa , germination , microcosm , bacillus (shape) , microbial inoculant , biology , spore , pythium ultimum , paenibacillus , microbiology and biotechnology , botany , biological pest control , bacteria , horticulture , inoculation , ecology , genetics , 16s ribosomal rna
Antagonistic endospore‐forming Bacillus spp. offer a large potential as seed inoculants for control of soil‐borne pathogens. In the soil, however, inoculated Bacillus endospores may remain dormant without germination, and plant protection can therefore be inefficient and unpredictable. A method based on direct fluorescence microscopy in soil microcosms was used to determine whether low‐cost organic additives incorporated into seed coating material could stimulate endospore germination. Complex organic additives supported a high level of endospore germination of the fungal antagonist Paenibacillus polymyxa CM5‐5. Skim milk is a low‐cost additive that may be incorporated into seed coating material for efficient induction of Bacillus endospore germination in soil.