Premium
Physiology and taxonomy of Thiobacillus strain TJ330, which oxidizes carbon disulphide (CS 2 )
Author(s) -
Hartikainen T.,
Ruuskanen J.,
Räty K.,
Von Wright A.,
Martikainen P.J.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01150.x
Subject(s) - library science , wright , chemistry , computer science , history , art history
A bacterium (strain TJ330) capable of using carbon disulphide (CS 2 ) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS 2 and hydrogen sulphide (H 2 S) from air. Its physiology and taxonomy are described here. The strain oxidized CS 2 , H 2 S and elemental sulphur to sulphate chemolithotrophically. The rate of sulphate production was highest at pH 2. The maximum growth rate constant (µ max ) using CS 2 as a substrate was 3·9 × 10 −2 h −1 (generation time 18 h) and the Monod constant (K s ) was 0·97–2·6 µmol l −1 CS 2 (74–198 µg l −1 ), corresponding to an equilibrium with 15–40 ppm CS 2 in the headspace. The optimum growth temperature using elemental sulphur as a substrate was 28 °C. The strain bears morphological and physiological similarities to Thiobacillus thiooxidans, but the latter is incapable of oxidizing CS 2 . The strain TJ330 (DSM 8985) showed only 44·2 + 11·8% DNA homology with the type strain T. thiooxidans ATCC 19377, while its homology with T. ferrooxidans ATCC 23270 was 17·1 + 3·4%. The strain TJ 330 represents a high‐affinity bacterium which can effectively remove low CS 2 concentrations in an acid environment. These properties can be utilized in biotechnological purification applications.