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16S to 23S rRNA spacer fragment length polymorphism of Salmonella enterica at subspecies and serotype levels
Author(s) -
Christensen H.,
Møller P.L.,
Vogensen F.K.,
Olsen J.E.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01095.x
Subject(s) - salmonella enterica , serotype , biology , subspecies , spacer dna , salmonella , ribosomal rna , typing , internal transcribed spacer , 23s ribosomal rna , genetics , ribosomal dna , 16s ribosomal rna , microbiology and biotechnology , polymerase chain reaction , gene , bacteria , phylogenetics , ribosome , paleontology , rna
The variation in the lengths of the internal transcribed spacer (ITS) between 16S and 23S rRNA genes of 101 strains representing 58 serotypes of Salmonella enterica (used for Salm. choleraesuis ) subsp. enterica (I), salamae (II), arizonae (IIIa), diarizonae (IIIb), houtenae (IV) and indica (VI) was used for typing by PCR amplification. Ten fragment lengths were observed by denaturing polyacrylamide gel electrophoresis on an automatic DNA sequencer resulting in 21 unique fragment patterns. Ten out of the 58 serotypes showed specific patterns but 48 serotypes were not fully differentiated. More than one ITS pattern was observed in seven serotypes. Five of the 21 fragment patterns contained representatives of more than one subspecies. Under non‐denaturing electrophoresis conditions, serotype specificity was obtained but precise ITS fragment length determination was not possible. DNA sequence comparison between ITSs of individual rrn operons is needed to further interpret ITS diversity within Salm. enterica at serotype and subspecies levels.