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High expression of the penicillin G acylase gene ( pac ) from Bacillus megaterium UN1 in its own pac minus mutant
Author(s) -
Panbangred W.,
Weeradechapon K.,
Udomvaraphant S.,
Fujiyama K.,
Meevootisom V.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01093.x
Subject(s) - bacillus megaterium , bacillus subtilis , mutant , bacillaceae , plasmid , mutagenesis , microbiology and biotechnology , bacillales , strain (injury) , penicillin , gene , chemistry , biology , bacteria , biochemistry , antibiotics , genetics , anatomy
By marker exchange mutagenesis, Bacillus megaterium strain UN‐1 (Bm‐UN1) was used to prepare a mutant strain B. megaterium UN‐cat (Bm‐UNcat) lacking the penicillin G acylase gene ( pac ). The pac gene from Bm‐UN1 was subcloned into pTF6 and the resultant plasmid, pBA402, was introduced into Bm‐UNcat and Bacillus subtilis . Bm‐UNcat harbouring pBA402 produced high penicillin G acylase (PAC) activity of 13·7, 19·5 and 20·4 U ml −1 at 24, 36 and 48 h of culture, respectively. This was two‐ to fivefold higher than PAC produced by B. subtilis harbouring pBA402 and about 20‐fold higher than PAC produced by the parent strain, Bm‐UN1.