Premium
Production of catalase and glucose oxidase by Aspergillus niger using unconventional oxygenation of culture
Author(s) -
Fiedurek J.,
Gromada A.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01085.x
Subject(s) - catalase , hydrogen peroxide , glucose oxidase , aspergillus niger , oxygen , bioreactor , chemistry , limiting oxygen concentration , oxygenation , mycelium , biochemistry , food science , chromatography , biology , enzyme , botany , organic chemistry , ecology
A novel method for increasing dissolved oxygen concentration in culture media has been developed. It involves adding hydrogen peroxide (H 2 O 2 ) to the medium which is then decomposed to oxygen and water by catalase. Some factors affecting oxygenation of culture were investigated. Maximal oxygen concentration occurred in 50 ml of the medium containing 0·2 g wet mycelium and 0·2% glucose at pH 5·0. A new apparatus for automated addition of H 2 O 2 to the bioreactor to keep the dissolved oxygen concentration constant over the range 1–100% ± 2% was tested. A significant increase (over sixfold) of intracellular catalase activity was obtained while the dissolved oxygen concentration remained stable (30% ± 2%).