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Chitinases from Vibrio : activity screening and purification of chiA from Vibrio carchariae
Author(s) -
Suginta W.,
Robertson P.A.W.,
Austin B.,
Fry S.C.,
FothergillGilmore L.A.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01076.x
Subject(s) - chitinase , chitin , vibrio alginolyticus , microbiology and biotechnology , vibrionaceae , vibrio , biology , agar , agar plate , enzyme , biochemistry , chitosan , bacteria , genetics , gene
Fourteen species of Vibrio were screened for chitin‐induced chitinase activity in culture medium. V. carchariae , V. alginolyticus 283 and V. campbellii showed high levels of activity. Screening on agar plates containing swollen chitin showed high levels of chitinase activity by the same three species, and also by V. fischeri and V. alginolyticus 284. An affinity purification procedure was developed for the chitinase from V. carchariae . The purified chitinase was active as a monomer with M r 63 000–66 000, and displayed activity toward polymeric chitin from acetylated chitosan or from crab shells. N‐terminal sequence analysis and immunological cross‐reactivity confirmed that the enzyme belongs to the group A/chiA family of bacterial chitinases.