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Evolution of a degradative bacterial consortium during the enrichment of naphtha solvent
Author(s) -
Cavalca L.,
Confalonieri A.,
Larcher S.,
Andreoni V.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01072.x
Subject(s) - naphtha , dioxygenase , pseudomonas putida , population , biology , biodegradation , aromatic hydrocarbon , microbiology and biotechnology , enrichment culture , strain (injury) , catechol , pseudomonadales , biochemistry , pseudomonas , chemistry , gene , bacteria , genetics , demography , anatomy , sociology , catalysis , ecology
A microbial mixed culture able to degrade naphtha solvent, a model of hydrocarbon aromatic mixture, was isolated from a hydrocarbon‐polluted soil. Composition of the population was monitored by phenotypic and molecular methods applied on soil DNA, on whole enrichment culture DNA, and on 85 isolated strains. Strains were characterized for their 16S rDNA restriction profiles and for their random amplified polymorphic DNA profiles. Catabolic capabilities were monitored by phenotypic traits and by PCR assays for the presence of the catabolic genes methyl mono‐oxygenase ( xyl A,M), catechol 2,3 dioxygenase ( xyl E) and toluene dioxygenase ( tod C1) of TOL and TOD pathways. Different haplotypes belonging to Pseudomonas putida , Ps. aureofaciens and Ps. aeruginosa were found to degrade aromatic compounds and naphtha solvent. The intrinsic catabolic activity of the microbial population of the polluted site was detected by PCR amplification of the xyl E gene directly from soil DNA.