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Susceptibility testing: accurate and reproducible minimum inhibitory concentration (MIC) and non‐inhibitory concentration (NIC) values
Author(s) -
Lambert R.J.W.,
Pearson J.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01017.x
Subject(s) - gompertz function , minimum inhibitory concentration , limiting , function (biology) , inhibitory postsynaptic potential , value (mathematics) , chemistry , mathematics , chromatography , statistics , biology , in vitro , biochemistry , mechanical engineering , evolutionary biology , neuroscience , engineering
Measuring the minimum inhibitory concentration (MIC) of a substance by current methods is straightforward, whereas obtaining useful comparative information from the tests can be more difficult. A simple technique and a method of data analysis are reported which give the experimentalist more useful information from susceptibility testing. This method makes use of a 100‐well microtitre plate and the analysis uses all the growth information, obtained by turbidometry, from each and every well of the microtitre plate. A modified Gompertz function is used to fit the data, from which a more exact value can be obtained for the MIC. The technique also showed that at certain concentrations of inhibitor, there was no effect on growth relative to a control well (zero inhibitor). Above a threshold value, which has been termed the non‐inhibitory concentration or NIC, growth becomes limiting until it reaches the MIC, where no growth relative to the control is observed.