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Characterization of Bacillus anthracis strains used for vaccination
Author(s) -
Cataldi A.,
Mock M.,
Bentancor L.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.01005.x
Subject(s) - bacillus anthracis , library science , vaccination , medicine , biology , virology , computer science , genetics , bacteria
Three Bacillus anthracis strains, formerly used as anti‐anthrax vaccine strains in Argentina, were characterized from genetic and pathogenic perspectives. Southern blotting and PCR with pXO1 and pXO2 probes and primers, as well as pathogenicity and protection tests in guinea pigs and mice, were performed. Two of the B. anthracis strains contained both pXO1 and pXO2 plasmids, as did the fully virulent strains, while the third was a Sterne‐type strain (pXO1 + , pXO2 – ). The three strains were, however, markedly less pathogenic than a wild‐type virulent strain. The methodology applied here may be used to characterize other B. anthracis strains .