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Purification and some characteristics of enterocin ON‐157, a bacteriocin produced by Enterococcus faecium NIAI 157
Author(s) -
Ohmomo S.,
Murata S.,
Katayama N.,
Nitisinprasart S.,
Kobayashi M.,
Nakajima T.,
Yajima M.,
Nakanishi K.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.00866.x
Subject(s) - enterococcus faecium , library science , political science , computer science , biology , bacteria , genetics
S. OHMOMO, S. MURATA, N. KATAYAMA, S. NITISINPRASART, M. KOBAYASHI, T. NAKAJIMA, M. YAJIMA and K. NAKANISHI.2000. Bacteriocin‐like activity (BLA) was screened in 690 strains of lactic acid bacteria isolated from plant materials such as silages and fermented vegetables. Among them, a strain identified as Enterococcus faecium NIAI 157 showed a clear BLA against the indicator strain, Ent. faecium IFO 13712. The proteinaceous nature and antimicrobial activity against closely related species strongly indicated that this BLA was a bacteriocin and was designated enterocin ON‐157. The bacteriocin activity of this strain was extracellularly produced in the logarithmic growth phase in MRS broth and purified by ultrafiltration, ammonium sulphate precipitation and cation‐exchange chromatography. Purified enterocin ON‐157 had a molecular weight of approximately 2500 Da in SDS‐PAGE analysis and was easily inhibited by treatment with α‐amylase and proteolytic enzymes. Enterocin ON‐157 had a bactericidal mode of action and inhibited the growth of the enterococci, Lactobacillus sake and Listeria monocytogenes . Enterococcus faecium NIAI 157 harboured two plasmids, 49·0 kb and 43·7 kb, and a variant missing a larger plasmid by curing with novobiocin lost the bactriocin activity.