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Growth kinetics of Acetobacterium sp. on methanol‐formate in continuous culture
Author(s) -
Bainotti A. E.,
Nishio N.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.00854.x
Subject(s) - formate , methanol , chemistry , yield (engineering) , fermentation , methyl formate , metabolism , kinetics , nuclear chemistry , biochemistry , catalysis , organic chemistry , materials science , physics , quantum mechanics , metallurgy
The fermentative metabolism of Acetobacterium sp. grown on methanol–formate in continuous culture is described. The reaction stoichiometry of methanol‐formate, including cells, were as follows: CH 3 OH + 1·13HCOOH → 0·87CH 3 COOH + 0·47 cell C. Formate enhanced growth yields by approximately 60% compared with methanol–CO 2 ‐grown cultures. Comparison of yields on methanol–formate allowed calculation of an energy yield of 1·3 mol ATP per mol acetate formed during homoacetate fermentation. The magnitudes of Y EG ,the theoretical maximum yield of Y E , and m , the maintenance coefficient, were determined by growing the organism in methanol–formate and resulted in 16·5 g cell (mol methanol catabolized) −1 and 0·674 mmol methanol catabolized (g cell) −1 h −1 , respectively. It is concluded that formate might replace CO 2 as a source of carboxyl donor.