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Virulence of Vibrio parahaemolyticus isolated from cultured small abalone, Haliotis diversicolor supertexta , with withering syndrome
Author(s) -
Liu P.C.,
Chen Y.C.,
Huang C.Y.,
Lee K.K.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.2000.00843.x
Subject(s) - abalone , biology , microbiology and biotechnology , vibrio parahaemolyticus , haliotis , virulence , agar , vibrio , desiccation , bacteria , vibrionaceae , hemolysin , agar plate , food science , fishery , botany , biochemistry , genetics , gene
Outbreaks of mass mortality among cultured small abalone Haliotis diversicolor supertexta with withering syndrome occurred in May and September 1998 in Kao‐Hsiung, Taiwan. Bacterial strains CH‐1 and B4 were isolated from the haemolymph of the moribund small abalone using tryptic soy agar supplemented with 3% NaCl and/or thiosulphate citrate bile salt sucrose agar. These two strains were characterized and identified as Vibrio parahaemolyticus on the basis of various biochemical tests. The B4 strain and its extracellular products were virulent to small abalone with LD 50 values of 1·6 × 10 5 colony‐forming units and 7·58 µg protein g −1 body weight, respectively.