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Inhibitory effect of intestinal anti‐Gb3 IgA antibody on verotoxin‐induced cytotoxicity
Author(s) -
Watarai S.,
Inoue Ta K.,
Oguma K.,
Naka K.,
Kodama H.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.2000.00836.x
Subject(s) - cytotoxicity , antibody , liposome , immune system , immunostimulant , immunoglobulin a , chemistry , immunology , adjuvant , pharmacology , biology , immunoglobulin g , biochemistry , in vitro
The effect of intestinal IgA antibody against the receptor for verotoxin (VT), globotriaosylceramide (Gb3), on VT‐mediated cytotoxicity was examined. Intestinal IgA antibodies against Gb3 were prepared by oral immunization of mice with Gb3 and adjuvant monophosphoryl lipid A (MPL)‐containing liposomes composed of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylserine and cholesterol (1 : 1 : 2, molar ratio) (PS‐liposome). Oral administration with Gb3 and MPL‐containing PS‐liposome induced significant IgA responses to Gb3 in the intestinal lavage fluid in all mice tested. Furthermore, anti‐Gb3 IgA antibodies in the lavage fluid effectively inhibited the cytotoxicity of VT2 to Vero cells in a dose‐dependent manner. These results suggest that anti‐Gb3 IgA antibodies produced in the intestinal tract, upon oral immunization with Gb3‐containing liposome, function as inhibitors against VT and also indicate the potential usefulness of oral PS‐liposome vaccines containing MPL for the induction of a protective mucosal immune response against intestinal diseases.