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Influence of sodium chloride on the β‐glucuronidase activity of Clostridium perfringens and Escherichia coli
Author(s) -
Fujisawa T.,
Aikawa K.,
Takahashi T.,
Yamai S.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.2000.00812.x
Subject(s) - clostridium perfringens , public health , political science , library science , chemistry , medicine , biology , computer science , bacteria , genetics , nursing
While the β‐glucuronidase activity of intact cells of Clostridium perfringens was higher in 0·95% sodium chloride (NaCl) than that in 0, 0·1 or 0·5%, that of Escherichia coli was higher in 0·1% NaCl than that in 0, 0·5 or 0·95% NaCl in 0·1 mol l −1 KH 2 PO 4 . However, the enzyme activity of both species of intact cells was higher in buffer containing 16 mEq sodium, 134 mEq potassium and 16 mEq chloride per litre than in that containing 146 mEq sodium, 13 mEq potassium and 146 mEq chloride. These findings suggest that bacterial cells are affected by the presence of NaCl and that the effect of NaCl on the activity of bacterial β‐glucuronidase may differ by location in the large intestine.