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Spatial distribution of 16S rRNA levels from uncultured acidobacteria in soil
Author(s) -
Felske A.,
De Vos W.M.,
Akkermans A.D.L.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.2000.00780.x
Subject(s) - acidobacteria , library science , german , geography , biology , 16s ribosomal rna , archaeology , computer science , bacteria , genetics , proteobacteria
The activity of uncultured acidobacteria was monitored in Dutch grassland soils by quantifying their ribosomes. These bacteria were detectable by five different 16S rRNA RT‐PCR products in temperature gradient gel electrophoresis fingerprints. The ribosomes in surface soil samples were quantified with multiple competitive RT‐PCR along a 1·5‐km transect through the grassland. In total, the five members of the acidobacteria were estimated to contribute 4 × 10 10 to 1 × 10 11 ribosomes g soil −1 , representing 7–14% of all bacterial ribosomes. These results indicate that ribosomes from acidobacteria are continuously present and abundant in soil and might contribute significantly to microbial activity in soil.