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Rapid detection of Staphylococcus aureus using bioluminescent enzyme immunoassay
Author(s) -
Fukuda S.,
Tatsumi H.,
Igarashi H.,
Igimi S.
Publication year - 2000
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.2000.00779.x
Subject(s) - staphylococcus aureus , microbiology and biotechnology , immunoassay , bioluminescence , biotinylation , coagulase , micrococcaceae , biology , enzyme , protein a , bacteria , staphylococcus , luciferase , biochemistry , antibody , immunology , genetics , transfection , gene
A bioluminescent enzyme immunoassay (BLEIA) method for detecting protein A‐bearing Staphylococcus aureus was developed using biotinylated firefly luciferase. The BLEIA was able to detect protein A at one pg ml −1 and 10 3 cfu ml −1 level of Staph. aureus . The BLEIA showed significant signals with overnight cultures of all 24 Staph. aureus strains, and the BLEIA did not show any significant signals with overnight cultures of all 44 strains of coagulase‐negative staphylococci and the other genus bacteria. After 5 h cultivation beginning at ≈ 50 cfu ml −1 , the BLEIA was able to detect all 35 Staph. aureus strains isolated from healthy humans.