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A method for bacteriocin quantification
Author(s) -
Cabo M. L.,
Murado M. A.,
González M. P.,
Pastoriza L.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00942.x
Subject(s) - bacteriocin , nisin , leuconostoc mesenteroides , parameterized complexity , incubation , extraction (chemistry) , chemistry , chromatography , biology , microbiology and biotechnology , computational biology , biological system , bacteria , computer science , biochemistry , antimicrobial , algorithm , lactic acid , genetics
M.L. CABO, M.A. MURADO, M.P. GONZÁLEZ and L. PASTORIZA.1999.Different aspects of the most commonly used assay methods in the study of bacteriocins were examined. The conditions under which extraction and incubation (including exposure time) take place were analysed, and several different formal models that are usually employed to calculate ID 50 were compared. As an alternative designed to overcome the problems which characterize the response of micro‐organisms that are sensitive to bacteriocins, an operative procedure in a liquid medium and a modified re‐parameterized logistic equation is proposed. When applied to the inhibition of Leuconostoc mesenteroides by nisin, the model allows an optimal experimental procedure to be defined.