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Effect of process temperature, pH and suspended solids content upon pasteurization of a model agricultural waste during thermophilic aerobic digestion
Author(s) -
Ugwuanyi J. O.,
Harvey L. M.,
McNeil B.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00831.x
Subject(s) - serratia marcescens , pasteurization , thermophile , enterococcus faecalis , digestion (alchemy) , food science , anaerobic digestion , chemistry , microorganism , mesophile , slurry , population , dilution , microbiology and biotechnology , escherichia coli , bacteria , biology , chromatography , biochemistry , environmental science , environmental engineering , enzyme , methane , physics , sociology , gene , thermodynamics , genetics , demography , organic chemistry
Thermophilic aerobic digestion(TAD), or liquid composting, is a versatile new process for the treatment and stabilization of high strength wastes of liquid or, perhaps more importantly, slurry consistency. The pattern of inactivation of various pathogenic and indicator organisms was studied using batch digestions under conditions that may be expected to be found in full‐scale TAD processes. Rapid inactivation of test populations occurred within the first 10 min from the start of digestion. The inactivation rate was slightly lower when digestions were conducted below 60 °C. In some instances, a ‘tail’ was apparent, possibly indicating the survival of relatively resistant sub‐populations particularly in the case of Serratia marcescens and Enterococcus faecalis , or of clumping or attachment of cells to particulate materials. The effect of pH on the inactivation of the test populations depended on the temperature of digestion, but varied with the test population. At 55 °C Escherichia coli was more sensitive to temperature effects at pH 7 than at pH 8, but was more sensitive at pH 8, 60 °C. The reverse was the case at 60 °C for Ent. faecalis . An increase in the solid content of the digesting waste caused a progressive increase in the protection of test organisms from thermal inactivation. Challenging a TAD process with test strains allows (via estimation of D ‐values) a quantification of the cidal effects of such processes, with a view to manipulating process variables to enhance such effects.