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Laboratory‐scale inactivation of African swine fever virus and swine vesicular disease virus in pig slurry
Author(s) -
Turner C.,
Williams S. M.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00802.x
Subject(s) - slurry , african swine fever virus , virus , chemistry , nuclear chemistry , virology , biology , materials science , composite material
Two methods were evaluated for the inactivation of African swine fever (ASV) and swine vesicular disease (SVD) viruses in pig slurry: chemical treatment and heat treatment. The addition of NaOH or Ca(OH) 2 at different concentration/time combinations at 4 °C and 22 °C was examined, as was virus stability at different temperature/time combinations. ASF virus (ASFV) was less resistant to both methods than SVD virus (SVDV). In slurry from one source, ASFV was inactivated at 65 °C within 1 min, whereas SVDV required at least 2 min at 65 °C. However, it was found that thermal inactivation depended on the characteristics of the slurry used. Addition of 1% (w/v) of NaOH or Ca(OH) 2 caused the inactivation of ASFV within 150 s at 4 °C; 0·5% (w/v) NaOH or Ca(OH) 2 required 30 min for inactivation. NaOH or Ca(OH) 2 (1% (w/v)) was not effective against SVDV at 22 °C after 30 min, and 1·5% (w/v) NaOH or Ca(OH) 2 caused inactivation of SVDV at both 4 °C and 22 °C. At higher chemical concentrations or temperatures, ASFV and SVDV inactivation was faster in slurry than in buffered medium.