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Electrotransformation of Lactobacillus manihotivorans LMG 18010 T and LMG 18011
Author(s) -
Sanoja R. Rodriguez,
MorlonGuyot J.,
Guyot J. P.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00800.x
Subject(s) - library science , biology , art , computer science
An electroporation procedure was developed for the genetic transformation of intact cells of Lactobacillus manihotivorans , a new Lactobacillus species isolated from cassava sour starch fermentation in Colombia. Transformation efficiency of Lact. manihotivorans strains LMG 18010 T and LMG 18011 was measured and compared with electroporability of Lact. plantarum strains NCIMB 8299 and LMG 9211, by investigating the effect of changes in various parameters. For Lact. manihotivorans strain LMG 18010 T , the composition of the culture medium, such as the type of peptone and the presence of Tween‐80, was found to be the most critical parameter, as well as the aeration conditions of the culture; better electroporation was obtained without air. The presence of MgCl 2 in the recovery medium was favourable to regeneration of electroporated cells. Plasmid‐curing of the cells did not improve their electroporability. Transformants were obtained with Lact. manihotivorans strain LMG 18010 T and the plasmids pLZ12 and pGK13, whereas Lact. manihotivorans strain LMG 18011 was transformable with plasmids pLP825 and pLZ12, with different electroporation conditions.