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Production of B‐group vitamins by two Rhizobium strains in chemically defined media
Author(s) -
Sierra S.,
Rodelas B.,
MartínezToledo M. V.,
Pozo C.,
GonzálezLópez J.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00765.x
Subject(s) - library science , humanities , art , computer science
Twenty‐eight strains of Rhizobium spp. were tested for their ability to grow in chemically‐defined medium lacking growth factors. Two strains, R. meliloti GR4B and Rhizobium spp. ( Acacia ) GRH28, were selected, on the basis of their good growth under the conditions imposed, for further quantification of the production of water‐soluble vitamins (thiamine, niacin, riboflavin, pantothenic acid and biotin) in chemically defined media amended with different compounds (mannitol, glucose or sodium succinate) as sole carbon sources. Qualitative and quantitative production of vitamins in chemically‐defined media was significantly affected by the use of C sources of a different nature and the age of the cultures. Strain GRH28 produced all the vitamins analysed, and high biological levels of biotin (14 ng ml –1 culture) were detected after 6 d of culture in mineral medium amended with mannitol. Pantothenic acid was the vitamin detected in the highest amounts (up to 1 μg ml –1 of culture) in culture supernatant fluids of strain GR4B grown for 6 d with succinate as sole carbon source.