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Inositol polyphosphate‐mediated iron transport in Pseudomonas aeruginosa
Author(s) -
Hirst P. H.,
Riley A. M.,
Mills S. J.,
Spiers I. D.,
Poyner D. R.,
Freeman S.,
Potter B. V. L.,
Smith A. W.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00697.x
Subject(s) - polyphosphate , pseudomonas aeruginosa , inositol , microbiology and biotechnology , pseudomonadales , chemistry , pseudomonadaceae , biochemistry , bacteria , biology , phosphate , genetics , receptor
It has previously been shown that myo ‐inositol hexakisphosphate ( myo ‐InsP 6 ) mediates iron transport into Pseudomonas aeruginosa and overcomes iron‐dependent growth inhibition. In this study, the iron transport properties of myo ‐inositol trisphosphate and tetrakisphosphate regio‐isomers were studied. Pseudomonas aeruginosa accumulated iron (III) at similar rates whether complexed with myo ‐Ins(1,2,3)P 3 or myo ‐InsP 6 . Iron accumulation from other compounds, notably d / l myo ‐Ins(1,2,4,5)P 4 and another inositol trisphosphate regio‐isomer, d ‐ myo ‐Ins(1,4,5)P 3 , was dramatically increased. Iron transport profiles from myo ‐InsP 6 into mutants lacking the outer membrane porins oprF, oprD and oprP were similar to the wild‐type, indicating that these porins are not involved in the transport process. The rates of reduction of iron (III) to iron (II) complexed to any of the compounds by a Ps. aeruginosa cell lysate were similar, suggesting that a reductive mechanism is not the rate‐determining step.