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Differentiation between brewing and non‐brewing yeasts using a combination of PCR and RFLP
Author(s) -
Yamagishi H.,
Otsuta Y.,
Funahashi W.,
Ogata T.,
Sakai K.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00691.x
Subject(s) - brewing , biology , saccharomyces cerevisiae , saccharomyces , polymerase chain reaction , yeast , restriction fragment length polymorphism , gene , genetics , food science , fermentation
In order to differentiate brewing from non‐brewing yeasts, a specific polymerase chain reaction (PCR) which targeted the open reading frame of FLO1 was employed. Non‐brewing yeasts include ‘non‐brewing Saccharomyces yeasts’ and ‘non‐ Saccharomyces yeasts’. The molecular sizes of the PCR products differed between brewing and non‐brewing Saccharomyces yeasts. No FLO1 PCR products were obtained from non‐ Saccharomyces yeasts. Specific PCR, using oligonucleotide primers that targeted the region between the 5S and 26S rRNA genes, could be used to differentiate brewing yeasts from some non‐brewing yeasts. These PCR products were digested with restriction enzymes, Scr FI and Msp I. Different restriction profiles were obtained from brewing and non‐brewing yeasts which could not be differentiated using specific PCR of rDNA. These results suggest that it is possible to identify brewing from non‐brewing yeasts using specific PCR of FLO1 and rDNA, and detection of restriction fragment polymorphism of rDNA.