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Identification of Rhizobium leguminosarum and Rhizobium sp. ( Cicer ) strains using a custom Fatty Acid Methyl Ester (FAME) profile library
Author(s) -
Dunfield K. E.,
Xavier L. J. C.,
Germida J. J.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00637.x
Subject(s) - rhizobium leguminosarum , biology , rhizobium , rhizobiaceae , biovar , rhizobia , bacteria , microbiology and biotechnology , botany , symbiosis , genetics
The potential of using fatty acid methyl ester (FAME) profiles of Rhizobium leguminosarum bv. viceae , phaseoli and trifolii , and Rhizobium sp. ( Cicer ) strains, for the identification of unknown isolates was assessed. This was achieved by developing a Rhizobium FAME library using 16 different Rhizobium strains of Rh. leguminosarum bv. viceae ( n = 5), Rh. leguminosarum bv. phaseoli ( n = 5), Rh. leguminosarum bv. trifolii ( n = 1) and Rhizobium sp. ( Cicer ) ( n = 5). Although there were considerable differences between Rh. leguminosarum biovars and strains and Rhizobium sp. ( Cicer ) strains, the variation within a particular biovar of Rh. leguminosarum was not high. Nevertheless, the feature FAME profiles of the various groups in the library allowed 75 putative rhizobia obtained from surface‐sterilized nodules of field‐grown lentil and pea plants to be identified.