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Efficient subtyping of Yersinia enterocolitica bioserotype 4/O:3 with pulsed‐field gel electrophoresis
Author(s) -
FredrikssonAhomaa M.,
Autio T.,
Korkeala H.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00625.x
Subject(s) - pulsed field gel electrophoresis , xhoi , subtyping , yersinia enterocolitica , biology , gel electrophoresis , microbiology and biotechnology , genotype , yersinia , genetics , bacteria , restriction enzyme , gene , computer science , programming language , ecori
The purpose of this study was to evaluate the efficiency of pulsed‐field gel electrophoresis (PFGE) for differentiation of isolates of Yersinia enterocolitica bioserotype 4/O:3 recovered from pig tongues at retail level in the Helsinki area during October and November of 1996. Not I generated 15 different PFGE patterns for 128 isolates. The discriminatory index did not exceed 74% due to the presence of two predominant PFGE‐types, NA1 (58/128) and NB1 (25/128). After preliminary investigations with 35 enzymes, Apa I, Xba I, Xho I and Spe I were chosen for further characterization of the isolates. The discriminatory index increased from 74% to 93% and the number of different genotypes from 15 to 30 when isolates with the same PFGE pattern with Not I were further characterized with Apa I and Xho I, indicating that PFGE can be an efficient technique for characterization of bioserotype 4/O:3.