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ppGpp accumulation in Pseudomonas aeruginosa and Pseudomonas fluorescens subjected to nutrient limitation and biocide exposure
Author(s) -
Greenway D. L. A.,
England R. R.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00615.x
Subject(s) - pseudomonas fluorescens , pseudomonas aeruginosa , pseudomonadales , pseudomonadaceae , microbiology and biotechnology , biocide , bacteria , pathogen , biology , pseudomonas , western blot , chemistry , biochemistry , gene , genetics , organic chemistry
The effect of a commonly used biocide, 1,2‐benzisothiazolin‐3‐one (BIT) on ppGpp accumulation in the pathogen, Pseudomonas aeruginosa PAO1, and an environmental isolate, Ps. fluorescens , was examined. It is concluded that BIT is able to induce a stringent response in Ps. aeruginosa and Ps. fluorescens , determined by the rapid accumulation of ppGpp following addition of BIT to exponentially‐growing cells. Western blot analysis of whole‐cell extracts with anti‐RelA antibody demonstrated that both species contain a RelA homologue. This is the first report of a RelA‐like protein in pseudomonads.