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Cell surface display of hepatitis B virus surface antigen by using Pseudomonas syringae ice nucleation protein
Author(s) -
Kim E. J.,
Yoo S. K.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00612.x
Subject(s) - pseudomonas syringae , library science , bioproducts , microbiology and biotechnology , virology , medicine , biology , computer science , pathogen , biofuel
A new system designed for cell surface display of recombinant proteins on Escherichia coli was evaluated for expression of eukaryotic viral antigens. The major surface antigen of hepatitis B virus (HBsAg) was fused to the ice nucleation protein (INP), an outer membrane protein of Pseudomonas syringae . Western blotting, immunofluorescence microscopy, whole‐cell ELISA, and ice nucleation activity assay confirmed expression of recombinant proteins on the surface of Escherichia coli . This study indicated that INP‐based cell surface display can be used for epitope mapping and recombinant bacteria expressing hepatitis viral antigens may be used for developing live vaccines.