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Genetic homogeneity and phage susceptibility of ruminal strains of Streptococcus bovis isolated in Australia
Author(s) -
Klieve A. V.,
Heck G. L.,
Prance M. A.,
Shu Q.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00596.x
Subject(s) - streptococcus bovis , biology , microbiology and biotechnology , restriction enzyme , strain (injury) , streptococcus , endonuclease , bacteria , dna , homogeneous , streptococcaceae , virology , genetics , rumen , antibiotics , food science , anatomy , fermentation , physics , thermodynamics
The genetic homogeneity of 37 strains of ruminal streptococci was investigated by comparing DNA fragment profiles on agarose gels following restriction endonuclease digestion with Hae III, Cfo I and Msp I. Thirty strains were indistinguishable from Streptococcus bovis strains, 2B, H24 and AR3. The remaining three strains were similar but not identical to a ruminal strain of Strep. intermedius (AR36). In addition, the susceptibility of these strains to infection by five bacteriophages was examined. Three of the phages (φSb02, φSb03 and φSb04) were specific to the strain of Strep. bovis from which they were isolated, while phages 2BV and φSb01 infected one and two strains, respectively, in addition to their primary host. It was concluded that although Strep. bovis is relatively homogeneous genetically, broad host range phages appear to be uncommon with this bacterial species.