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Cloning and sequencing of the gene encoding universal stress protein from Escherichia coli O157:H7 isolated from Jack‐in‐a‐Box outbreak
Author(s) -
Chen J.,
Griffiths M. W.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00594.x
Subject(s) - escherichia coli , gene , biology , homology (biology) , genetics , nucleic acid sequence , peptide sequence , base pair , cloning (programming) , microbiology and biotechnology , computer science , programming language
A gene encoding the universal stress protein ( uspA ) was cloned from Escherichia coli O157:H7 isolated from a Jack‐in‐a‐Box outbreak. The nucleotide sequence was determined and the amino acid sequence predicted. The gene was found to be highly conserved and shared 97% homology with the same gene of E. coli K‐12. Within an 800‐base pair (bp) region sequenced, a 15‐bp mismatch existed between E. coli K‐12 and O157:H7, of which 11 base pairs were internal to the structural gene of uspA . However, only an area with a 4‐bp mismatch caused an amino acid substitution, changing an alanine to an arginine at position 140.