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Enhanced sporulation in Bacillus subtilis grown on medium containing glucose:ribose
Author(s) -
Warriner K.,
Waites W. M.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00593.x
Subject(s) - spore , pentose phosphate pathway , bacillus subtilis , ribose , sporogenesis , biochemistry , biology , glycolysis , metabolism , bacteria , chemistry , microbiology and biotechnology , enzyme , genetics
Spore crops of Bacillus subtilis PS 346 were increased by the addition of a combination of glucose and ribose to the sporulating medium. The increase in spore yield was over 100% higher in glucose:ribose‐containing cultures, compared with cells grown on glucose as a sole carbon and energy source. Spore crops obtained from cultures grown on glucose:ribose had similar thermal resistance and hydrodynamic mean radius to those obtained when cultivated solely on glucose‐ or ribose‐containing media. When other combinations of dual carbohydrates were tested it was apparent that those substrates primarily channelled through the pentose phosphate pathway gave enhanced sporulation. This effect was also observed by supplementing glucose‐containing media with pyruvate but not citrate or malate. Those substrates that are channelled through the glycolytic pathway were also less effective in this respect. From the results obtained it appears that the enhancement of sporulation could be attributed to alteration in carbon and electron flow through metabolic pathways leading, directly or indirectly, to metabolites that play a role in sporogenesis regulation.