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Colicin V can be produced by lactic acid bacteria
Author(s) -
McCormick J. K.,
Klaenhammer T. R.,
Stiles M. E.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00571.x
Subject(s) - colicin , bacteriocin , bacteria , escherichia coli , microbiology and biotechnology , enterobacteriaceae , biology , signal peptide , lactic acid , peptide , peptide sequence , biochemistry , genetics , gene
Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram‐positive bacteria. Export of colicin V is dependent on specific ABC (ATP‐binding cassette) secretion proteins which recognize a double‐glycine‐type leader peptide on the immature colicin V bacteriocin. Replacement of the colicin V leader peptide by a signal peptide from the signal sequence‐dependent bacteriocin divergicin A allowed expression of colicin V in lactic acid bacteria. This system may serve as a model for the heterologous expression of other small bacteriocins active against Gram‐negative bacteria and other antibacterial peptides from lactic acid bacteria.