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Detection of IgG antibody to Bovine Leukaemia Virus in urine and serum by two enzyme immunoassays
Author(s) -
Carli K. T.,
Sen A.,
Batmaz H.,
Kennerman E.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00561.x
Subject(s) - antibody , urine , virology , enzyme , immunoassay , bovine serum albumin , virus , immunoglobulin g , biology , microbiology and biotechnology , chemistry , immunology , biochemistry
Four hundred blood sera from a cattle production unit were tested for BLV‐(Bovine Leukaemia Virus) antibody with IP (Institut Porquier) and SB (Svanova Biotech) ELISA kits. Seventy‐seven cattle with BLV‐antibody (19·25%) and 77 without the antibody were used. No significant difference was found between O.D. of sera of PL + (Persistent Lymphocytosis Positive) and PL– (Negative) cattle. The mean O.D. of urine samples of 77 seropositive cattle was significantly higher than that of 77 seronegative cattle ( P <0·01). There were also differences between urine O.D.s of seropositive (PL + ) and seropositive (PL–) groups of cattle with IP ( P <0·05) and SB ( P <0·01) kits. All the results revealed the presence of BLV‐antibody in the urine of the cattle without any urinary dysfunction.