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Formation of a chiral acetoinic compound from diacetyl by Escherichia coli expressing meso ‐2,3‐butanediol dehydrogenase
Author(s) -
Ui S.,
Mimura A.,
Ohkuma M.,
Kudo T.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00560.x
Subject(s) - 2,3 butanediol , acetoin , escherichia coli , diacetyl , dehydrogenase , chemistry , strain (injury) , isopropyl , enzyme , yield (engineering) , stereochemistry , biochemistry , biology , medicinal chemistry , fermentation , gene , materials science , anatomy , metallurgy
l ‐Acetoin ( l ‐AC) was produced from diacetyl (DA) by Escherichia coli JM109/pBUD119 containing the meso ‐2,3‐butanediol dehydrogenase ( d ‐AC forming) gene. However, when the strain was cultured in the presence of isopropyl‐β‐ d ‐thiogalacto‐pyranoside, the enzyme formed catalysed not only d ‐AC but also l ‐AC. l ‐AC was further reduced to l ‐2,3‐butanediol (BD). The yield of l ‐AC or l ‐BD from DA (3 g l −1 ) was about 70% (w/w).