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An easy colorimetric assay for screening and qualitative assessment of deiodination and dehalogenation by bacterial cultures
Author(s) -
Kuritz T.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00538.x
Subject(s) - iodine , halogenation , iodide , biotransformation , chemistry , colorimetry , bacteria , chromatography , environmental chemistry , combinatorial chemistry , organic chemistry , biochemistry , biology , enzyme , genetics
Halogenated organic substances are among the main environmental concerns. A number of micro‐organisms are able to dehalogenate these compounds. However, the methods for the assessment of micro‐organismal ability to dehalogenate are expensive and require complex instrumentation. Here, an easy colorimetric assay for the screening and assessment of the ability of bacterial cultures to deiodinate, and potentially dehalogenate, chemical substances is proposed. The method is based on the oxidation of iodide, released due to biotransformation, to iodine followed by a subsequent detection of iodine by a classical reaction with starch.