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Evaluation of a molecular identification scheme based on 23S rRNA gene polymorphisms for differentiating canine and feline gastric Helicobacter spp.
Author(s) -
Jalava K.,
Hielm S.,
Hirvi U.,
Hänninen M. L.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00527.x
Subject(s) - 23s ribosomal rna , biology , restriction fragment length polymorphism , felis , helicobacter , ribosomal rna , helicobacter pylori , 16s ribosomal rna , genetics , gene , terminal restriction fragment length polymorphism , restriction enzyme , polymerase chain reaction , microbiology and biotechnology , cats , rna , ribosome , computer science , embedded system
A scheme for the rapid identification of Helicobacter spp. using restriction fragment length polymorphism digestion profiles of PCR amplified 23S rRNA genes is described. The efficacy of this scheme for speciation of the closely related gastric species H. felis , H. bizzozeronii and H. salomonis was evaluated. It was difficult to distinguish between some RFLP profiles obtained and often, more than one profile was seen with each species examined. Some evidence was found that the 23S rRNA gene copies of these species may not be identical. Moreover, the identification scheme was ineffective in discriminating these species from each other, although they could be differentiated, as a group, from other Helicobacter spp. The results indicate that this scheme should be carefully evaluated with a number of isolates if it is to be applied to additional, highly related Helicobacter spp.