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Abundance and distribution of bacteria carrying sltII gene in natural river water
Author(s) -
Kurokawa K.,
Tani K.,
Ogawa M.,
Nasu M.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00516.x
Subject(s) - bacteria , biology , primer (cosmetics) , gene , escherichia coli , microbiology and biotechnology , chemistry , genetics , organic chemistry
Direct in situ PCR with HNPP/Fast Red TR was used to enumerate bacteria carrying the sltII gene in river water. By direct in situ PCR with a sltII ‐specific EVS primer, 10 2 –10 5 cells ml −1 of bacteria carrying the sltII gene were detected from all sampling sites, except the site nearest to the source of the river, while 10 2 –10 4 cells ml −1 of Escherichia coli O157:H7 were detected using a direct fluorescent antibody staining method. These results indicate that such bacteria are commonly distributed in natural river water. Direct in situ PCR with HNPP/Fast Red TR is a useful tool for detecting cells carrying specific genes, such as verotoxin‐producing bacteria in natural environments.