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Identification of a gene encoding an immuno‐reactive membrane protein from Campylobacter jejuni
Author(s) -
Connerton P. L.,
Connerton I. F.
Publication year - 1999
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1365-2672.1999.00491.x
Subject(s) - campylobacter jejuni , open reading frame , biology , gene , nucleic acid sequence , snap23 , microbiology and biotechnology , genetics , campylobacter coli , escherichia coli , peptide sequence , hspa2 , bacteria
A gene encoding a putative membrane protein has been identified from Campylobacter jejuni NCTC 11168 following an immuno‐screen of a lambda ZAP II genomic DNA library with antiserum raised against glycine‐extractable proteins. The nucleotide sequence of the entire genomic insert revealed six open reading frames, all but one of which have sequence homologues in the complete genome sequence of Helicobacter pylori . The gene encoding the immuno‐reactive protein was further identified by independent expression of these reading frames in Escherichia coli. The gene encodes an integral membrane protein, expression of which in E. coli results in a profound filamentous phenotype.